Introduction

This software is intended for the acquisition of image stacks (movies) for single molecule imaging experiments utilizing total internal reflection fluorescence (TIRF) microscopes and wide-field sCMOS cameras. It is designed to be used with the SPARTAN data analysis software.

Please cite the following paper if you use FLASH in your laboratory:

Juette MF, Terry DS, Wasserman MR, Altman RB, Zhou Z, Zhao H, Blanchard SC. Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale. *Nature Methods*. 2016 Apr;13(4):341-4. doi: 10.1038/nmeth.3769.

This software is intended only for research use in an academic setting. For licensing information, please see “license.txt” file associated with the software.

For a list of recent changes with this version, please see “release.txt” file associated with the software.

If you have comments, concerns, bug reports, or feature requests, please contact Scott Blanchard (scott.blanchard@stjude.org) or visit our GitHub page.